Otsu-juku was the last of the sixty-nine stations of the Nakasendo, as well as the last of the fifty-three stations of the Tokaido. It was 14 km (9 mi) from the previous post town, Kusatsu-juku, and was located in Omi Province. It is currently located in the present-day city of Otsu, Shiga Prefecture, Japan.
Otsu-juku was a rather large post town, with approximately 350 homes, two honjin, a secondary honjin and 71 inns for other travelers.